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Department of Biology
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![]() RNA interference can be used to disrupt gene expression in Tribolium. The T. castaneum pu-11 enhancer trap line expresses fluorescent GFP protein in the eyes and other tissues. This can be eliminated by injection of dsRNA encoding GFP into pu-11 larvae. The reduced expression level in the resulting adults is comparable to wildtype, which lack GFP activity entirely.
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Copyright 2009 David R. Angelini